Isolation and Culture of Mouse Mesenchymal Stem Cells (MSCs)

1. Sacrifice 6-10 weeks old mice by CO2.

2. Isolate the bones and flush out bone marrow with a 5 ml syringe through a 25 gauge needle

    with antibiotics enriched medium

3. transfer the marrow cell suspension to six well plates at a concentration of 1.5x106 nucleated cells per cm2

4. Culture the cell in low glucose DMEM with 10% FBS.

5. After 72 hours, remove the nonadherent cells and renew half of the medium.

6. Trypsinize, harvest, and passage the cells, once the adherent cells reach 70-80% confluence.

7. Passage 3-5 homogenous cells can be used for other experiment.

Protocols

1. Culture of Human Embryonic Stem Cells (hESC)
2. Establishment and characterization of human embryonic stem cell line
3. Rat stem cell isolation and culture 
4. Mouse stem cell isolation and culture 
5. Induction of Stem Cell Differentiation   
6. Cancer stem cells
7. Video Data on Stem Cell Research (click to show)