Introduction to Elisa: Enzyme-Linked ImmunoSorbent Assay, or ELISA, is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality control check in various industries. In simple terms, in ELISA an unknown amount of antigen is affixed to a surface, and then a specific antibody is washed over the surface so that it can bind the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal. Thus in the case of fluorescence ELISA, when light is shone upon the sample, any antigen/antibody complexes will fluoresce so that the amount of antigen in the sample can be measured.
Protocol 2. Indirect Elisa Protocol
Protocol 2. Detection
of recombinant proteins by Elisa
Protocol 3. The procedure for optimized Elisa Protocol
Protocol 4. Enzyme-Linked Immunosorbent
Assay (ELISA) Protocol
Protocol 5. Double antibody sandwich Elisa (DAS-Elisa)
Protocol 6. Cytokine Elisa Protocol
Protocol
7. Qualitative PCR?CELISA protocol for the detection and typing of viral genomes
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Ch 8.Immunohistoch / immunology
Ch 10.GC/MS, NMR and Proteomics