Microarray Protocols
Basic knowledge of microarray.
Basic knowledge of microarray 2.
MicroArray Procedure
(Modified by Ryoji Kojima, Takumi Yoshida and Jeffrey Randall,
8.20.2000)
1. Total RNA Isolation from cultured cells.
2. DNase Treatment of Total RNA
3. Making the single strand cDNA probe.
4. Automated
Slide Processor (ASP) Version for hybridization.
5. Washing microarrays in ASP.
7. Pre-hybridization of
the processed slides (NON-Automated version).
8. Hybridization of Cy3 + Cy5 probe to glass array (NON-Automated version).
9. Preparation
of Dendrimer Cy3 and Cy5.
10. Washing unbound probe from glass array (NON-Automated version).
11. Hybridization of Dendrimers (Cy3
and Cy5) to Array (NON-Automated version).
12.Washing unbound dendrimer from glass array(NON-Automated version).
Other
Microarray Protocols (1)
Other Microarray Protocols (2)
Web Guider
Ch 8.Immunohistoch / immunology
Ch 10.GC/MS, NMR and Proteomics