In addition, protocols for conjugation of Annexin V to the following molecules are given as models for fluorescent conjugation of
non-immunoglobulin proteins. Conjugation of other fluorescent molecules would proceed very similarly to the protocols listed below
(use as a template the protocol Type that is appropriate for the molecule you are interested int).
You
may view the fluorescence spectra of the fluorescent molecules listed above (all together), or view individual fluorescence spectra
for:
You can also view absorbance spectra for:
Many
people have contributed to the successful conjugations that we routinely perform in the laboratory. In particular, Aaron Kantor designed
and optimized many of the basic protocols referenced in these pages, and made the chemical conjugations simple for all of the biologists
here. Randy Hardy and Alan Stall devised the original reductive cross-linking protocols used for all the protein-protein conjugations.
Other people who have been helpful in the development of the more recent protocols include Masahiko Amano, Michael Anderson, Stephen
DeRosa, Rachel Gerstein, Peter Katsikis, Gina Jager, and Iwan Tjioe.
These pages were designed and written by Mario Roederer.
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