3 M Sodium acetate (pH 5.2)(1 liter)

1.      Weigh out 408.1 g sodium acetate.3H2O.

2.     Add 800 ml distilled water to dissolve.

3.      Adjust to pH 7.0 with dilute acetic acid.

4.     Make up volume to 1 liter with distilled water.

5.      Dispense into aliquots and sterilize by autoclaving.
<STORAGE> Store at room temperature.

 

5 M NaCl (1 liter)

1.      Weigh out 292.2 g NaCl.

2.     Add 800 ml distilled water to dissolve.

3.      Make up volume to 1 liter with distilled water.

4.     Dispense into aliquots and sterilize by autoclaving.
<STORAGE> Store at room temperature.

 

10% Sodium dodecyl sulphate (SDS) (also called sodium lauryl sulphate)(1 liter)

1.      Weigh out 100g of electrophoresis-grade SDS.
<CAUTION> Wear a mask when weighing SDS and wipe down the weighing area and balance after use because the fine crystals of SDS disperse easily.

2.      Add 900 ml distilled water and heat to 68 C to dissolve.

3.      Adjust to pH 7.2 using concentrated HCl.

4.      Make up volume to 1 liter with distilled water.

5.     Dispense into aliquots. There is no need to sterilize 10% SDS.
<STORAGE> Store at room temperature.

 

20 x SSC (salt, sodium citrate)(1 liter)

1.      Weigh out 175.3 g NaCl and 88.2 g sodium citrate.

2.      Add 800 ml distilled water to dissolve.

3.      Adjust to pH 7.0 using 10 M NaOH.

4.     Make up volume to 1 liter with distilled water.

5.      Dispense into aliquots and sterilize by autoclaving.
<STORAGE> Store at room temperature.

 

20 x SSPE (salt, sodium phosphate, EDTA)(1 liter)

1.      Weigh out 175.3 g NaCl, 31.2 g NaH2PO4.2H2O and 7.4 g EDTA.

2.      Add 800 ml distilled water to dissolve.

3.     Adjust to pH 7.4 with 10 M NaOH (about 6.5 ml).

4.      Make up volume to 1 liter with distilled water.

5.     Dispense into aliquots and sterilize by autoclaving.
<STORAGE> Store at room temperature.

 

Trichloroacetic acid (TCA) 100% solution (500 g TCA)

1.      Add 227 ml distilled water to a bottle containing 500 g of TCA.
<STORAGE> Store at room temperature.

 

1 M Tris (1 liter)

1.      Weigh out 121.1 g Tris base.

2.     Add 800 ml distilled water to dissolve.
<CRITICAL> If the 1 M solution has a yellow colour, discard it and obtain better quality Tris.

3.      Adjust the pH to the desired value by adding concentrated HCl. For a pH of 7.4 add 70 ml HCl; for a pH of 7.6 add 60 ml HCl; and for a pH of 8.0 add 42 ml HCl.
<CRITICAL> Many types of electrodes do not accurately measure the pH of Tris solutions, but suitable electrodes can be obtained from most manufacturers.
<CRITICAL> Allow the solution to cool to room temperature before making final adjustments to the pH. The pH of Tris solutions is temperature-dependent and decreases approximately 0.03 pH units for each 1 C increase in temperature.

4.      Make up volume to 1 liter with distilled water.

5.      Dispense into aliquots and sterilize by autoclaving.
<STORAGE> Store at room temperature.

 

Tris-buffered saline (TBS) (25 mM Tris)(1 liter)

1.      Weigh out 8 g NaCl, 0.2 g KCl and 3 g Tris base.

2.     Add 800 ml distilled water to dissolve.

3.      Add 0.015g phenol red.

4.      Adjust to pH 7.4 with HCl.

5.      Make up volume to 1 liter with distilled water.

6.     Dispense into aliquots and sterilize by autoclaving (20 minutes at 15 lb in-2 on liquid cycle).
<STORAGE> Store at room temperature.

 

Tris-acetate for electrophoresis (TAE)(50X)(1 liter)

1.      Weigh out 242 g Tris base.

2.     Add 800 ml distilled water to dissolve.

3.      Add 57.1 ml glacial acetic acid and 100 ml 0.5 M EDTA (pH 8.0)

4.      Make up volume to 1 liter with distilled water.
<STORAGE> Store at room temperature.
Dilute 1:49 with distilled water. TAE has a rather low buffering capacity, and tends to become exhausted during extended electrophoresis. Replacement of the buffer or recirculation between the two reservoirs is therefore advisable when carrying out electrophoresis for long periods of time at high current.

 

Tris-borate for electrophoresis (TBE)(5X)(1 liter)

1.      Weigh out 54 g Tris base and 27.5 g boric acid.

2.      Add 800 ml distilled water to dissolve.

3.     Add 20 ml 0.5 M EDTA (pH 8.0)

4.      Make up volume to 1 liter with distilled water.
<STORAGE> Store at room temperature in glass bottles. Discard any batches containing precipitate.
Dilute 1:4 or 1:9 with distilled water. Almost all agarose gel electrophoresis is now carried out with a 1:9 dilution of the concentrated stock. TBE is used at a working strength of 1 x for polyacrylamide gel electrophoresis as the buffer reservoirs of the vertical tanks used for polyacrylamide gel electrophoresis are fairly small, and the amount of electric current passed through them is often considerable.

 

 

 

Commonly used solutions (1)---  1 ) 2 ) 3 ) 4 ) 5 ) 6 )

 

Commonly used solutions (2)--- 1 ) 2 ) 3 )

 

Solutions  for molecular cloning---1 ) 2 ) 3 ) 4 ) 5 ) 6 ) 7 ) 8 ) 9 )