Streptomycin stock solution (20 mg ml-1)(50 ml)

1.      Weigh out 1 g streptomycin sulfate and dissolve in 45 ml of distilled water.

2.      Sterilise by filtration.
<STORAGE> Aliquots of appropriate volume can be stored at -20 C.
<USAGE> For culture plates, allow media to cool to 55 C before adding streptomycin to a final concentration of 25 ug/ml.

 

Tetracycline stock solution (12.5 mg ml-1)(80 ml)

1.      Weigh out 1 g tetracycline and dissolve in 75 ml of 1:1 vol/vol distilled water:ethanol.

2.      Make up volume to 80 ml with 1:1 vol/vol distilled water:ethanol.

3.      Sterilise by filtration.
<STORAGE> Aliquots of appropriate volume should be wrapped in aluminium foil and stored at -20 C.
<USAGE> For culture plates, allow media to cool to 55 C before adding streptomycin to a final concentration of 12.5-15 ug/ml. Store plates in the dark at 4 C. Magnesium ions are antagonists of tetracycline, therefore avoid using media, such as LB that contain magnesium salts.

 

Luria Broth (1 liter)

1.      Weigh out 25 g Luria broth powder and dissolve in 750 ml of distilled water.

2.      Add 800 ml distilled water to dissolve.

3.     Adjust to pH 7.0 with either HCl or NaOH as appropriate.

4.      Make up volume to 1 liter with distilled water.

5.      Sterilize by autoclaving for 15 min at 121 C.
<STORAGE> Store at 4 C.

 

M9 salts (10X)(1 liter)

1.      Measure 90 ml distilled water.

2.      Add 6.8 g Na2HPO4.7H2O, 3 g KH2PO4, 0.5 g NaCl and 1 g NH4Cl sequentially, allowing each salt to dissolve before adding the next.

3.     Make up volume to 100 ml with distilled water.

4.      Sterilize by autoclaving for 15 min at 121 C.
<STORAGE> Store at room temperature.

 

M9 Minimal Medium (1 liter)

1.      Weigh out 1 g NH4Cl and 3 g glucose.

2.     Add 100 ml 10X M9 Salts.

3.      Add 1 ml 1 M MgSO4 and 10 ml Gibco MEM vitamin solution.

4.     Make up volume to 1 liter with distilled water.

5.      Check that the pH is 7.0-7.4.

6.     Filter sterilize through a 0.22 micron filter.

7.      Add 1 ml sterile 100 mM CaCl2 solution.
<STORAGE> Store at room temperature.

 

 

Commonly used solutions (1)---  1 ) 2 ) 3 ) 4 ) 5 ) 6 )

 

Commonly used solutions (2)--- 1 ) 2 ) 3 )

 

Solutions  for molecular cloning---1 ) 2 ) 3 ) 4 ) 5 ) 6 ) 7 ) 8 ) 9 )