Nucleic Acid (DNA and RNA) Methods--Everything for DNA and RNA Research
 
 
  1. General Techniques for Handling Nucleic Acids
  2. DEPC treatment of solutions.
  3. Phenol Extraction.
  4. Ethanol Precipitation of Nucleic Acids
  5. PEG Precipitation of DNA
  6. Quantitation of DNA and RNA
  7. Restriction Enzyme Digestion
  8. Submarine Agarose Gels
  9. Southern Transfer of DNA Gels
  10. Random Primed Synthesis for the Preparation of Labeled DNA Probes
  11. Filter Hybridization Methods
  12. Guanidine Thiocyanate procedure for RNA extraction.
  13. Preparation of frozen tissue for RNA Extraction
  14. Isolation of RNA by Phenol Extraction.
  15. Isolation of Messenger RNA
  16. RNA Slot Blots
  17. Formaldehyde Denaturing Gels for RNA
  18. Ribonuclease Protection Assay -- Using S1 Nuclease
  19. RNAse Protection Assay
  20. Riboprobe Synthesis (Stratagene)
  21. Riboprobe Synthesis (Promega)
  22. Synthesis of Unlabeled RNA
  23. Subcloning of DNA fragments
  24. Phosphatase Treatment of DNA Termini
  25. Blunting 5' Overhangs with Klenow
  26. 5?End Labeling
  27. Bacterial Transformation
  28. Transformation of Bacterial Cells by Electroporation
  29. Colony Hybridization
  30. Sanger Double-Stranded DNA Sequencing
  31. Creating cDNA Libraries
  32. Generation of High Titer Stocks
  33. Titering Phage Libraries or Stocks
  34. Screening Lambda Libraries with Radiolabeled Probes
  35. Rapid Plasmid Mini-Preps
  36. Large Scale Phage DNA Isolation
  37. Boiling Plasmid DNA Prep
  38. Alkaline Lysis Plasmid Preps
  39. "TEEN-AGE" PREP METHOD (25ml)
  40. Ten Minute Plasmid Miniprep
  41. Microwave Plasmid Mini-Prep Protocol
  42. Preparation of M13 Single Stranded DNA
  43. Preparation of Single Stranded DNA from Bluescript
  44. DNA Amplification Using PCR
  45. Oligonucleotide-directed Mutagenesis by the Overlap PCR Amplification
  46. Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
  47. Immuno-Screening of l expression libraries
  48. Maxim and Gilbert DNA sequencing
  49. Fusion Protein Expression in E. coli
  50. Purification of His Tagged RMLC
 
 

Web Guider

Ch 1.General Lab Techniques

Ch 2.Molecular Separation

Ch 3.DNA and RNA

Ch 4.Genetics

Ch 5.PCR Serials

Ch 6.Protein

Ch 7.DNA Protein Interactions

Ch 8.Immunohistoch / immunology

Ch 9.Cellular Biology

Ch 10.GC/MS, NMR and Proteomics

Ch 11.Animal Experiments

Ch 12.Worm: C. Elegans

Ch 13.HPLC and TLC

Ch 14.Buffers formats in Lab.

Ch 15.Other Resources

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