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Southwestern Blotting

 

Background: Southwestern blotting was first described by Bowen et al. (1) and was used to identify DNA-binding proteins that specifically interact with a chosen DNA fragment in a sequence-specific manner. In this technique, mixtures of proteins such as crude nuclear extracts or partially purified preparations are first fractionated on a sodium dodecyl sulfate (SDS) denaturing gel; the gel is then equilibrated in a SDS-free buffer to remove detergent and the proteins transferred by electroblotting to an immobilizing membrane. During the transfer the proteins renature and hence DNA-binding proteins may subsequently be detected on the membrane by their ability to bind radiolabeled DNA. Fractionation of crude nuclear extracts on an SDS gel followed by electroblotting and analysis for sequence-specific DNA binding directly on the blot combines the advantages of a high-resolution fractionation step with the ability to rapidly analyze for a large number of different DNA-binding specificities.

 

Southwestern Bot-1

Protocol S.3. Southwestern Blot. This protocol is adopted from the Lambda gt22a screening protocol (L.1) and the protein gels protocol P.1. Solutions ...


genetics.med.harvard.edu/~cepko/protocol/mike/S3.html

Southwestern blotting in investigating transcriptional regulation ...

Southwestern blotting is useful in investigating DNA¨Cprotein interactions to ..... Southwestern blotting showed two bands on the blot hybridized with the ...
www.nature.com/nprot/journal/v3/n1/full/nprot.2007.492.html

 

An improved method for southwestern blotting, 1997]

We have developed a modified Southwestern blotting technique which utilizes .... Figure 1: (A) Southwestern blot of HL-60 nuclear extract probed with a ...
www.bioscience.org/1997/v2/c/handen/9-11.htm

 

South western blot mapping: a procedure for simultaneous characterization of DNA binding proteins and their specific genomic target sites ...

A method called "South Western blot mapping" for rapid characterization of both DNA binding proteins and their specific sites on genomic DNA is described. ...
www.ncbi.nlm.nih.gov/pubmed/2774177

 

Southwestern blot - Wikipedia, the free encyclopedia

Southwestern blotting, based along the lines of Southern blotting (which was created by Edwin Southern) and first described by B. Bowen and colleagues in ...
en.wikipedia.org/wiki/Southwestern_blot

 

Protocols of Southwestern & Footprint (PARP Lab)

Southwestern blot Material : 0.22 um BA83 or BAS83 nitrocellulose membrane (Schleicher & Schuell) [32P]-NAD = 1 uCi/ml (NEN Dupont de Nemours, ref. ...
parplink.u-strasbg.fr/protocols/tools/southwestern.html

 

Springer Protocols: Abstract: Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting ...

Southwestern blotting was first described by Bowen et al. ( 1 ) and was used to identify DNA-binding proteins that specifically interact with a chosen DNA ...
www.springerprotocols.com/Abstract/doi/10.1385/1-59259-208-2:255

 

Selection of sequences recognized by a DNA binding protein using a preperative southwestern blot...

We have tested the feasibility of using a preparative scale Southwestern blot to select DNA sequences specifically recognized by a DNA binding protein. ...
www.pubmedcentral.nih.gov/articlerender.fcgi?artid=328708

 

Identification of a New Response Element-binding Factor by Southwestern blotting ...

utilized the Southwestern blotting technique which can pro- ..... Southwestern blot of nuclear proteins resolved by one- and two-dimensional electophoresis ...
www.jbc.org/cgi/reprint/268/26/19581.pdf

 

Southern Blot

Other blotting methods (i.e., western blot, northern blot, southwestern blot) that employ similar principles, but using RNA or protein, have later been ...
www.molecularstation.com/dna/southern-blot/

 

Springer Protocols: Viral Protein - Nucleic Acid Interaction - Southwestern blot ...

2.5 South-Western Blot; 2.6 Radioactive Probe Stri... 2.7 Western Blotting; 3 Methods; 3.1 Preparation of the MSV... 3.2 SDS-PAGE Analysis ...
www.springerprotocols.com/Abstract/doi/10.1007/978-1-59745-102-4_28

 

Southwestern blot mapping of potential regulatory proteins binding to the DNA Encoding Plasminogen Activator Inhibitor Type 2 ...

We have explored the application of Southwestern blot mapping as an approach for identifying specific DNA-protein interactions and targeting potential ...
www.ihop-net.org/UniPub/iHOP/pm/107639.html?pmid=8262378

 

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