Developed in 1983 by Kary Mullis, PCR is now a common technique used in medical and biological research labs for a variety of tasks, such as the sequencing of genes and the diagnosis ofhereditary diseases, the identification of genetic fingerprints (used in forensics and paternity testing), the detection and diagnosis of infectious diseases, and the creation of transgenic organisms. Mullis won the Nobel Prize for his work on PCR.

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FRQs for PCR and other experiments on molecular biology

17. What is PCR?
18. What are some good references for PCR?
19. How should I select a set of primers to use for PCR?
20. What kinds of programs are available for designing PCR primers?
21. What is "Hot-start" PCR?
22. What is AP-PCR or RAPD PCR?
23. What is "Touchdown" PCR?
24. Is there a simple method to sequence lambda, M13, or plasmid clones using PCR?

34. Should we break up the methods-reagnts group into subsets with one being
exclusively on the polymerase chain reaction (PCR)?

In situ PCR: protocols and applications

Polymerase Chain Reaction (PCR) Animation

More PCR Protocols Online