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Molecular Biology / PCR / Variants of PCR / Long PCR Protocol

 

Kyle¡¯s protocol for long PCR

Kyle¡¯s protocol for long PCR. This protocol intended for generation of long PCR products (3-10kb) from mouse ES cell or tail DNA. General Considerations: ...
www.whitelabs.org/Lab%20Protocols/PCR%20Protocols/kyle.htm

 

Long PCR Protocol

Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School,Boston, MA, USA.
arep.med.harvard.edu/labgc/estep/longPCR_protocol.html

 

Tips for Long and Accurate PCR
Tips for Long and Accurate PCR. Wayne M. Barnes. Department of Biochemistry and Molecular Biophysics, Washington University School of ...
http://www.klentaq.com/products/lapcrchapter.pdf

 

Mullins Lab : Protocols : Long PCR

Two long PCR steps:. First round of the nested PCR step of the end point dilution procedure to quantitate the cDNA. First round of the nested PCR step of ...
ubik.microbiol.washington.edu/protocols/other/longpcr.htm

 

Long PCR

LONG PCR AMPLIFICATION OF THE FVIII GENE INTRON 22 GENE INVERSION. Introduction. Long range PCR allows the amplification of PCR products, which are much ...
europium.csc.mrc.ac.uk/WebPages/Database/Methods/longpcr.htm

 

Extra-Long PCR Ki, EPICENTRE Biotechnologies

EPICENTRE develops and manufactures reagents and kits for genomics, proteomics, and RNA research. EPICENTRE's products are used worldwide in a broad range ...
www.epibio.com/item.asp?ID=306

 

Long PCR Protocol

Efficient Long-PCR results from the use of two polymerases: a non-proofreading polymerase is the main polymerase in the reaction, and a proofreading ...
wheat.pw.usda.gov/~lazo/methods/lazo/longpcr.html

 

Long PCR.

Long PCR. Cheng S, Chang SY, Gravitt P, Respess R. Department of Human Genetics, Roche Molecular Systems, Inc., Alameda, California 94501. Mesh Terms: ...
www.ncbi.nlm.nih.gov/pubmed/8208299

 

Long PCR Product Sequencing (LoPPS): a shotgun-based approach to sequence long PCR products.

Here we describe a practical procedure for sequencing long PCR products. The method relies on ultrasonic shearing of PCR products, resulting in fragments ...
www.nature.com/nprot/journal/v2/n2/abs/nprot.2006.453.html

 

Long PCR Protocol

Aug 10, 2003 ... Introduction ¡¤ Research Pages ¡¤ People involved Photo galleries Publications Other sample sites Education ¡¤ Links to other micobial ...
www.hawaii.edu/microbiology/MO/longpcr.htm

 

Long PCR -- Sambrook and Russell.

Primers used for long PCR are generally slightly longer (25-30 nucleotides) than those used for standard PCR. It is particularly important to strive for ...
www.cshprotocols.org/cgi/content/full/2006/2/pdb.prot3841

 

Efficient Cloning of Long PCR Inserts with the In-Fusion PCR Cloning System.
Clontech¡¯s In-Fusion PCR cloning method. provides superior results when applied to the. challenging task of cloning long PCR fragments. ...
www.clontech.com/images/ctq/APR07UPD/CR742319_LongPCR_IF_US.pdf

 

Nature Protocols: Long PCR amplification of the entire mitochondrial genome from individual helminths for direct sequencing.

In this article, we describe a practical long PCR approach for the amplification and subsequent sequencing of the entire mt genome from individual helminths ...
www.natureprotocols.com/2007/09/20/long_pcr_amplification_of_the.php

 

Long PCR - MyBio

Long PCR Reagents and GuidelinesLong PCR Reagents and Guidelines. ... General Guidelines for Long PCR Conditions and Enzyme Mixtures Efficient Long PCR ...
mybio.wikia.com/wiki/Long_PCR

 

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