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Ch 8.Immunohistoch / immunology
Ch 10.GC/MS, NMR and Proteomics
Molecular Biology / PCR / Variants of PCR / Nested PCR
What is nested PCR? Nested PCR is a variation of the polymerase chain
reaction (PCR), in that two pairs (instead of one pair) of PCR primers are used to amplify a fragment.
The first pair of PCR
primers amplify a fragment similar to a standard PCR. However, a second pair of primers called nested primers (as they lie / are nested
within the first fragment) bind inside the first PCR product fragment to allow amplification of a second PCR product which is shorter
than the first one.
The advantage of nested PCR is that if the wrong PCR fragment was amplified, the probability is quite low that
the region would be amplified a second time by the second set of primers. Thus, Nested PCR is a very specific PCR amplification.
PCR
amplification of cDNA segments by 2 stage nested PCR
protocol
NCBI
Standard Nested PCR Protocol
C. elegans Gene Knockout Project,
Vancouver Lab
Poison Primer Nested PCR Protocol
Moerman Lab.
Nested PCR and Sequencing of T-DNA Junctions in Arabidopsis
http://signal.salk.edu/T-DNArecovery.pdf
ECKER
LAB