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Ch 8.Immunohistoch / immunology
Ch 10.GC/MS, NMR and Proteomics
IMolecular Biology / PCR / Variants of PCR / In Situ PCR
The In Situ PCR:
(Amplification and Detection in a Cellular Context)
Ernest
F. Retzel, Katherine A. Staskus, Janet E. Embretson and Ashley T. Haase
Department of Microbiology,
Table of Contents
Introduction
Technical Aspects/Philosophy
Primer Design
Reaction Conditions
"Mechanics" of the Reaction
Methodology
1. Cultured Cells: Infection and Preparation.
2. Tissues: Pulmonary infection and preparation.
1.
Cultured Cells in Suspension.
2. Tissues on Slides.
C. Detection of Amplified DNA by in situ Hybridization
1. Solution amplification.
2.
Tissue Sections.
In Situ PCR on Plant Material (Bo Johansen)
Required materials
Required solutions
Tissue preparation
Pectinase
treatment
Protease treatment
Acetylation
DNase treatment
Reverse transcription
Thermal cycling
Detection
Mounting
Acrobat version of Poster
References
Other
ISPCR links
Botanical Institute
RT IN SITU PCR
TABLE OF CONTENTS
3.1.2. Definition of optimal protease digestion
3.1.3. Definitions of suboptimal
and over-digestion
3.1.5. Direct incorporation of the reporter nucleotide
4. A one step protocol for RT in
situ PCR
5.1. MMP and
TIMP expression in cervical cancer
In situ PCR: protocols and applications.
groups
have published protocols and data using in situ PCR techniques. (5). This article will discuss some of the basic concepts of in situ
PCR, the proto- ...
www.genome.org/cgi/reprint/4/4/S151.pdf
Co-labeling Using In Situ PCR: A Review
Figure 2 Background vs signal
with RT in situ PCR. This renal biopsy was analyzed for mRNA by RT in situ PCR. After digestion for 10 min in ...
www.jhc.org/cgi/reprint/49/11/1329.pdf
Protocols
for the : in situ: PCR-amplification and detection of mRNA and DNA sequencing.
In this protocol we describe the in situ PCR method
for the amplification of both DNA and mRNA targets [in situ reverse transcriptase-PCR (RT-PCR)], ...
www.nature.com/nprot/journal/v2/n11/abs/nprot.2007.395.htmlw.
96-well
RNA In Situ Hybridization Protocol
A protocol for PCR in situ hybridization
of hyphomycetes
Introduction. Within the last years the focus of mycological studies has shifted. emphasis from mere taxonomic studies
to more ecological. questions. ...
www.im.microbios.org/03setember98/08%20Sterflinger.pdf