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3' RACE PCR
This technique is used to obtain the 3''end of a cDNA. Protocol by R Hastings, Biochemistry, University of Nottingham Medical School, UK.
http://www.nottingham.ac.uk/~mbzspd/m...

A Rapid DNA Minipreparation Method Suitable for AFLP and Other PCR Applications (PDF document)
Preparation of DNA from plant tissues suitable for PCR methods including AFLP, article by DH CHEN and PC RONALD Department of Plant Pathology, University of California, Davis.
http://pubs.nrc-cnrc.gc.ca/ispmb/ispm...

Adjuvants in PCR Reactions
Brief discussion of additives to improve amplification efficiency and specificity of PCR, by Octavian Henegariu,Yale-New Haven Medical Center.
http://info.med.yale.edu/genetics/war...

Alu-PCR Hybridization
A report and protocol, from Fondation Jean Dausset.
http://www.cephb.fr/manips_alupcr.html

Anchor Probes for Comparative Mapping of Grass Species
Article in which probes from different libraries were used to hybridize seven cereals at the Department of Plant Breeding and Biometry, Cornell University, NY.
http://greengenes.cit.cornell.edu/anc...

Attotron Biosensor Corporation
R & D company for development of biosensors and related products for the research and educational markets.
http://www.attotron.com

Biosource molecular methods booklet
56 page booklet on oligonucleotides, PCR and RT-PCR, from Biosource (company).
http://www.biosource.com/content/tech...

C. elegans Gene Knockout Project
Protocols including standard nested PCR, from the Biotechnology Laboratory, University of British Columbia.
http://ko.cigenomics.bc.ca/

Cleaved Amplified Polymorphic Sequence
Illustration of principle. CAPS corresponds to RFLP. From Corvallis Forestry Research Community, Corvallis, USA.
http://www.fsl.orst.edu/tgerc/PCRCour...

DNALC: PCR Animation
An animation explaining how the Polymerase Chain Reaction (PCR) works, from the Dolan DNA learning center, Cold Spring Harbor Laboratory.
http://www.dnalc.org/shockwave/pcranw...

Degenerate PCR
An introduction with principle, applications and description of the technique. From the web site of Kebangsaan University, Malaysia.
http://cgat.ukm.my/protease/degpcr.html

Design Oligonunucleotide Primers
Interface for scanning DNA sequences for restriction sites, without changing encoded protein created. Includes FAQ, table of enzymes and links to tools, maintained at the University of Waterloo, ON, Canada.
http://sciborg.uwaterloo.ca/~mpalmer/...

Detection of Asymmetric PCR Products in Homogeneous Solution by Fluorescence Correlation Spectroscopy
In asymmetric PCR, the low concentration primer is quantitatively incorporated into double stranded DNA after an appropriate number of cycles. If this primer is fluorescent labelled, the dsDNA can be quantitated from the fluorescence.
http://imd-www.es.hokudai.ac.jp/~kinj...

Detection of Point Mutations by RFLP of PCR Amplified DNA Sequences
RFLP = Restriction Fragment Length Polymorphism. By Alexander Binder 1997.
http://www-ang.kfunigraz.ac.at/~binde...

Detection of Single Nucleotide Mutations in Wheat Using Single Strand Conformation Polymorphism Gels (PDF file)
P Martins-Lopez, H Zhang, R Koebner, Plant Mol. Biol. Reporter 19(2001): 159-162. From National Research Coouncil Canada.
http://pubs.nrc-cnrc.gc.ca/ispmb/ispm...

Effect of PCR Buffer on multiplex PCR (PDF)
Multiplex PCR employs different primer pairs in the same amplification reaction. This requires extensive optimization of annealing conditions. From Quiagen (company)
http://www.qiagen.com/literature/broc...

FISH Guide and Troubleshooting
Links to pages describing influential parameters, with guides on PCR, RT-PCR and multiplex PCR reactions, Taq, FISH, CM-FISH, TM-FISH, microarrays, CCK, slide prep and labeling, maintained by Octavian Henegariu from Yale University, New Haven, CT.
http://info.med.yale.edu/genetics/war...

Fidelity of DNA Polymerases for PCR
Article by PN Hengen from TIBS 1995
http://www.lecb.ncifcrf.gov/~pnh/pape...

GenHunter
Manufacturer of material for differential display PCR in Nashville,Tenn USA.
http://www.genhunter.com

HiFi DNA
HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails.
http://www.hifidna.com/

Improved diagnosis of porcine proliferative enteropathy caused by Lawsonia intracellularis using polymerase chain reaction-enzyme-linked oligosorbent assay
P Zhang, CJ Gebhart, D Burden, GE Duhamel: Molecular and Cellular Probes, 14(2) 101-108, on the site of BT&C, Inc.
http://www.btc-bti.com/pcrelisa.htm

Innovative Tools for Amplification
Notes about PCR applications, principles, research results, from Roche.
http://www.roche-applied-science.com/...

Inverse PCR For use with Snyder mTn-lacZ/LEU2 Based Mutagenesis
Protocol by M McMurray, Fred Hutchinson Cancer Research Center, Seattle, Wa. USA.
http://www.fhcrc.org/labs/gottschling...

Inverse PCR and Cycle Sequencing of P Element Insertions for STS Generation
Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA.
http://www.fruitfly.org/about/methods...

Inverse PCR for PAC-end sequencing
To generate PCR fragments that contain the ends of PAC inserts that can be sequenced. Protocol by B Barbazuk,Washington University Zebrafish Genome Resources Project, USA.
http://www.genetics.wustl.edu/fish_la...

Inverse PCR protocol
step by step protocol, from the web site of the Department of Biology, University of Michigan, USA.
http://www.biology.lsa.umich.edu/rese...

Kary B. Mullis - Autobiography
The originator of PCR, from the Nobel e-museum web site.
http://nobelprize.org/chemistry/laure...

Kary Mullis
Inventor Profile of Kary Mullis, the originator of PCR, from the National Inventors Hall of Fame web site.
http://www.invent.org/hall_of_fame/10...

Life at High Temperatures
Article by TD Brock from the web site of Department of Bacteriology, University of Wisconsin - Madison on the thermophilic organisms in Yellowstone hot springs, including Thermus aquaticus from which Taq polymerase was obtained.
http://www.bact.wisc.edu/Bact303/b27

Long PCR Protocol
Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept.,Harvard Medical School, Boston, MA, USA
http://twod.med.harvard.edu/labgc/est...

MJ Research
Manufactures and sells products for molecular biology including thermal cyclers and sequencers / genotypers.
http://www.mjr.com/

Molecular Staging, Inc.
Company promoting diagnostic tests for genetic analysis; rolling circle amplification technology (RCAT), developed for amplifying signals from DNA, RNA and proteins, from Westport, CT.
http://www.molecularstaging.com/Pages...

Mutation Detection Systems - SSCP Analysis
Discussion of the technique and variations, literature references. By S McQuaid, Institute of Child Health, University College London,UK.
http://www.ich.ucl.ac.uk/cmgs/sscp.htm

Nematode ITS1 Size Variation
Examples of Restriction Fragment Length Polymorphism (RFLP)electrophoresis slabs for different nematodes.
http://nematode.unl.edu/its_id/EXAMPL...

Optimizing Multiplex and LA-PCR with Betaine
LA-PCR = "long and accurate PCR". Article by PN Hengen in TIBS June 1997.
http://www-lecb.ncifcrf.gov/~pnh/pape...

Optimizing PCR Protocols
Brief guidelines. From the Jackson Laboratory, University of Maine, USA.
http://www.jax.org/imr/optimize_pcr.html

PCR Amplification of cDNA Segments by 2 Stage Nested PCR
Protocol from the method database of NIH, USA.
http://www.ncbi.nlm.nih.gov/SNP/snp_v...

PCR Project
Presentations from the University of California at Berkley on PCR, both current research reports and reviews.
http://sunsite.berkeley.edu/biotech/pcr/

PCR Protocol
Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.
http://www.biology.lsa.umich.edu/rese...

PCR Protocols
Protocols and technical hints, particularly for reverse transcription PCR, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Toxicology Program, Penn State University
http://www.cas.psu.edu/docs/CASDEPT/V...

PCR Technology
An introduction by Connie Veilleux from the US National Health Museum website.
http://www.accessexcellence.org/LC/SS...

PCR Troubleshooting
Limited to conventional straight forward PCR. Page designed and maintained by Octavian Henegariu on the web site of the Yale - New Haven Medical Center.
http://info.med.yale.edu/genetics/war...

PCR and multiplex PCR guide
Discussions of the parameters influencing the PCR reaction and some PCR and multiplex PCR applications, by Octavian Henegariu on the web site of the Yale - New Haven Medical center.
http://www.info.med.yale.edu/genetics...

PCR animated
Animation illustrating the principle of PCR, from the University of Ghent, Belgium.
http://allserv.rug.ac.be/~avierstr/pr...

PCR method protocols
Protocols for PCR posted by the Helen Donis-Keller Laboratory.
http://hg.wustl.edu/hdk_lab_manual/pc...

PCR primer design and reaction optimisation
Article by Ed Rybicki, Department of Molecular and Cell Biology, University of Cape Town in: Molecular Biology Techniques Manual, on the web site of the University of Cape Town.
http://web.uct.ac.za/microbiology/pcr...

PCR protocols
Brief protocols for PCR in use at the Genome Sequencing Center of the Washington University in St Louis Medical School.
http://genome.wustl.edu/tools/protoco...

Polymerase Chain Reaction
Popular survey article by Mark V. Bloom, DNA Learning Center, Cold Spring Harbor Laboratory, from the web site of the US National Health Museum.
http://www.accessexcellence.com/RC/CT...

Polymerase Chain Reaction (PCR)
A graphic description of the principle of PCR from the US National Health Museum web site.
http://www.accessexcellence.org/AB/GG...

Principle of PCR
Applications in work on aging of Caenorhabditis elegans and phylogeny of nematodes, by Andy Vierstraete, Department of Biology, University of Ghent, Belgium.
http://allserv.rug.ac.be/~avierstr/pr...

Protocol for T-RFLP by Capillary Electrophoresis
Detailed protocol for terminal restriction fragment length polymorphism (T-RFLP), which allows the fingerprinting of a community by analyzing the polymorphism of a certain gene. Article by V Grntzig, B Stres, HL Ayala del Ro and JM Tiedje, Center for Microbial Ecology, Michigan State University, USA
http://rdp.cme.msu.edu/html/t-rflp_ju...

Protocols Online: PCR Protocols
Extensive collection of PCR protocols and methods from Protocol On Line.
http://www.protocol-online.org/prot/M...

Quantitative PCR Protocol
From the Jackson Laboratory, University of Maine, USA.
http://www.jax.org/cyto/quanpcr.html

RACE amplification
Rapid Amplification of cDNA Ends. Protocol, from National Institute of Genetics, Japan.
http://www.nig.ac.jp/labs/EarlyEmb/mi...

RAPD PCR
RAPD stands for Random Amplification of Polymorphic DNA, where the target sequence(s) (to be amplified) is unknown.Brief description, fromRutgers University, USA.
http://avery.rutgers.edu/WSSP/Student...

RAPD analysis with P. infestans
Protocol for Randomly Amplified Polymorphic DNA, by H Judelson, University of California, Riverside, USA.
http://www.fgsc.net/methods/Judel.html

RAPD's
Brief description of Random Amplified Polymorphic DNA, from Oklahoma State University, USA.
http://opbs.okstate.edu/~melcher/MG/M...

RFLP Definition
RFLP = Restriction Fragment Length Polymorphism, from FDA
http://vm.cfsan.fda.gov/~frf/rflp.html

Random Arbitrarily Primed PCR
Protocol for RAP-PCR to study prokaryotic gene expression, from the Enterococcus Research Site, University of Oklahoma, USA.
http://www.enterococcus.ouhsc.edu/ran...

Rational primer design greatly improves differential display-PCR (DD-PCR)
Article: D Graf, AG Fisher, M Merkenschlager: Nucl. Acids Res. 25:11 2239-2240.
http://nar.oupjournals.org/cgi/conten...

Reference in PCR
Technical aspects of quantitative real-time PCR and RT-PCR. Instruments, kits, dyes, chemistries, and services presented by their manufacturers.
http://www.gene-quantification.org

Rep-PCR Genomic Fingerprinting
Bacteria are characterized by Rep-PCR fingerprinting using primers corresponding to naturally occurring repetitive sequences in the interspersed regions.
http://www.msu.edu/user/debruijn/

Roe Laboratory Protocols
Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK.
http://www.genome.ou.edu/proto.html

Single tube confirmation PCR protocol
For characterization colonies of transformed clones of Saccharaomyces, from the web site of the Stanford Genome Technology Center, Palo Alto, CA, USA.
http://www-sequence.stanford.edu/grou...

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