Proteomics Analysis Protocols (from GenWaybio)

Proteomics is the large-scale study of proteins, particularly their structures and functions. The proteome of an organism is the set of proteins produced by it during its life, in contrast to its genome as its set of genes. One organism has radically different protein expression in different parts of its body, different stages of its life cycle and different environmental conditions. Since proteins play a central role in the life of an organism, proteomics is instrumental in discovery of biomarkers and drug targets. To facilitate proteomics research, the following methods and approaches have been developed.

For further information on the technology and applications of proteomics analysis, as well as the related products and services, please visit the pages:

Sample Preparation
Protein Fractionation
SDS-PAGE or 2DE
Mass Spectrometry
Proteomic Identification and Profiling
Bioinformatics Analysis

GenWay offers solutions to proteomics research and sample preparation. For more detailed information, please see the specific description pages in Immunoaffinity Separation, Custom Antibodies, Immunoassay and Assay Development

Immunoaffinity Fractionation Protocols (from GenWay)

Separation or fractionation of complex protein mixtures that have an overwhelming dynamic range of concentration, such as plasma or serum, is an outstanding challenge for proteomic analysis. Sample preparation to remove highly-abundant proteins is an essential step for enhancing detection resolution by two-dimensional gel electrophoresis (2DE), liquid chromatography (LC) and/or mass spectrometry (MS). Various tools and methods for protein separation using affinity capture reagents are capable of specific binding and target removal. Among these reagents, immunoaffinity mediated separation of protein mixtures is the classical, natural, effective, and most reliable approach. Antigen affinity-purified polyclonal avian (chicken) IgY antibodies, compared to the more conventionally-used IgG antibodies, have unique and advantageous features that enable specific protein separation and sample preparation for low-abundant protein detection and biomarker discovery. To best meet the needs of proteomic analysis by 2DE and MS, it is desirable that sample preparation tools for protein separation or fractionation have the following features: High specificity and accuracy
Low cross-reactivity to non-target proteins
High avidity (strong binding power)
Bound proteins can be eluted and analyzed
High capacity (to process large volume per load)
Multi-species applicability
Convenient use (simple and easy operation)
Can be automated or streamlined
High reproducibility (with acceptable CV)
Good reusability (fidelity in regeneration)
Minimal disruption to natural condition of sample
Reasonable affordability (cost-effective) GenWay has developed the IgY-antibody microbeads (Seppro? technology by covalently coupling IgY antibodies to microbeads in a unidirectional fashion and mixing at an optimized ratio. This technology, compared to others on the market, is proprietary and represents a leading immunoaffinity separation product (Table 1)

Proteinics related protocols and databases (from Wikepedia, the free encyclopedia)