RNA Cloning Method Flowchart
MicroRNA and siRNA Cloning Method. Bartel Lab Protocol. Revised May, 2002 ... Oligos for RNA Cloning [Underline is Ban I site (G?GYRCC)] ...
web.wi.mit.edu/bartel/pub/protocols/miRNAcloning.pdf

MicroRNA and siRNA Cloning Protocol Bartel Lab Protocol Updated ...
Note: To synthesize this oligo in your lab, see the original 2001 version of the cloning protocol on the Bartel Lab website. 5' End Acceptor Oligo (RNA/DNA ...
web.wi.mit.edu/bartel/pub/protocols/miRNACloningUpdate0705.pdf -

Universal miRNA Cloning Linker (S1315), RNA Isolation and Analysis ...

Linker/RNA Ligation Protocol: The Universal miRNA cloning linker has a 3?blocking group (amine) to prevent self ligation, circularization and ligation to ...
www.neb.com/nebecomm/products/productS1315.asp

Ambion TechNotes 12(3): MicroRNA Cloning Overview

MicroRNA Discovery MicroRNA Cloning Overview. MicroRNAs (miRNAs) are an important class of small RNAs that regulate protein translation (see Getting Started ...
www.ambion.com/techlib/tn/123/6.html

Molecular Biology / miRNA microRNA protocols and methods

MicroRNA and siRNA Cloning Method new recommended protocol ... MicroRNA (miRNA) cloning protocol from Dr.Victor Ambros's lab at Dartmouth medical college. ...
biowww.net/browse-120.html

Cloning of artificial microRNAs
Cloning of artificial microRNAs. Protocol by Rebecca Schwab. MPI for Developmental Biology. Tuebingen, 2005. The artificial microRNA designer WMD delivers 4 ...
wmd2.weigelworld.org/themes/amiRNA/pics/Cloning_of_artificial_microRNAs.pdf

Nature Protocols: Cloning of amplified miRNA target sites into 3 ...

Before cloning, check miRNA target sequence for the presence of eventual restriction sites using for cloning. If it is the case, use another compatible ...
www.natureprotocols.com/2006/07/03/cloning_of_amplified_mirna_tar.php

pPRIME (Potent RNA interference using microRNA expression):
PNAS 2004) to ensure correct processing of the pre-miRNA by Drosha and Dicer. ... miR30-shRNA cloning protocol (for pSM2 and pPRIME) ...
elledgelab.bwh.harvard.edu/protocols/pPRIME/pPRIME_vectors.doc

Pre-made MicroRNA-Enriched cDNAs

The amplified cDNA was cloned following SBI’s protocol without a ... Among them, two clone appear to contain more than one microRNA-like sequences. ...
www.systembio.com/miRNALibrary.htm - 32k

A biochemical approach to identifying microRNA targets -- Karginov ...

Cloning of 3' UTRs. 3' UTR sequences were amplified by PCR and ligated into pENTR/D-TOPO (Invitrogen) following the protocol of the directional TOPO cloning ...
www.pnas.org/cgi/content/full/0709971104/DC1

Invitrogen - Epigenetics - Simple miRNA Labeling and Sensitive ...

Starting with a 250 ng to 5 ug total RNA sample, miRNAs are tagged directly in a quick and easy-to-use protocol with the NCode?Rapid miRNA Labeling System ...
www.invitrogen.com/content.cfm?pageid=12078

Cloning of short hairpin RNAs for gene knockdown in mammalian cells
EcoRI sites in the pSM2 miR-30 cloning cassette. The modified miR-30 context (Fig. 2) allows for. efficient processing of both the wild-type miR-30 miRNA ...
www.plantsci.cam.ac.uk/Haseloff/iGEM2006/PDFrefs/genetools/RNAas/files/Paddison2004.pdf

Isolating miRNA for Profiling Studies (Ambion TechNotes 13:2)

... nt from total RNA for subsequent miRNA array analysis or cloning applications. ... Fractionated samples are then concentrated using a 45 minute protocol ...
www.ambion.com/techlib/tn/132/5.html  

PCR scheme to produce artificial miRNA constructs from pNW55
Modification PCRs on template clone pNW55:. Primer. Product size. PCR protocol. G-4368 + primer II. 256 bp. Primer I + primer IV. 87 bp. Primer III + G-4369 ...
wmd2.weigelworld.org/themes/amiRNA/pics/modify_pNW55.pdf

MicroRNA methods

7: miRNA Microarray Protocol,113. 8: Data Analysis,118. Acknowledgments,119. References,119. 7: Cloning and Detecting Signature MicroRNAs from Mammalian ...
ntuml.mc.ntu.edu.tw/image-newbooks/2584304.htm

Real-Time PCR for MicroRNAS Tech Guide
cloning procedures. The entire microRNA sequence ...... Use a preset optimization protocol or a gradient protocol to decrease programming time ...
www.genomeweb.com/pdfs/RTPCRmicroRNATechGuide.pdf

RNAi Question and Answer Archive from the TRC

What vector can I clone the whole population into and how much starting material do I need to clone? A: Please refer to the detailed miRNA Cloning protocol ...
www.sigmaaldrich.com/.../Life_Science/Functional_Genomics_and_RNAi/Key_resources/RNAi_Answer_Archive.html

WMD - Web MicroRNA Designer::Help

Reference for determinants of plant microRNA target selection: Schwab et al. .... Simply order these primers and proceed according to the cloning protocol ...
wmd.weigelworld.org/cgi-bin/mirnatools.pl?page=6