BioProtocols--NMR 1

About Nuclear Magnetic Resonance Spectroscopy

Nuclear Magnetic Resonance (NMR) is not easy to describe in a few paragraphs, let alone understand. Therefore, this description of NMR takes a simplistic point of view. There is much more to NMR than is contained here.

The nuclei of certain atoms(hydrogen, for example) behave as if they are spinning like tiny, positively-charged tops. A spinning nucleus (because it is charged) generates its own magnetic field. If we place a sample containing these nuclei in an external magnetic field (between the poles of a powerful magnet) the magnetic field of the spinning nuclei can either be aligned with the external magnetic field, or opposed to it. It is more favorable for the nuclear magnetic field to be aligned with the external magnetic field.

The nucleus can change its orientation to the opposed state if it absorbs the right energy of radiofrequency radiation (energy from radio waves). The size of the energy difference between the two orientations is dependent on the strength of the magnetic field that the nucleus feels. Because molecular structures can shield or expose the atom from the external magnetic field, the energy of radiation absorbed provides information about the location of that nucleus within the molecule. In addition, adjacent nuclei interact to give us information about which nuclei are near one another.

Basics of NMR : A comprehensive treatment of NMR, includes some math and animation.

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