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Ch 1.General Lab Techniques

Ch 2.Molecular Separation

Ch 3.DNA and RNA

Ch 4.Genetics

Ch 5.PCR Serials

Ch 6.Protein

Ch 7.DNA Protein Interactions

Ch 8.Immunohistoch / immunology

Ch 9.Cellular Biology

Ch 10.GC/MS, NMR and Proteomics

Ch 11.Animal Experiments

Ch 12.Worm: C. Elegans

Ch 13.HPLC and TLC

Ch 14.Buffers formats in Lab.

Ch 15.Other Resources

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Biochemistry (Chapters and Vocabulary) -unit 1-50, 51-100, 101-150, 151-200, 201-250, 251-300, 301-350, 351-400, 401-450, 451-500

DNA Replication, Recombination, and Repair

1: Okazaki Fragments.                                                              

 

2: Priming.                                                           

 

3: Origin of Replication in E. coli.                                                        

 

4: Double-Stranded DNA Can Wrap Around Itself to Form Supercoiled Structures          

 

5: Topoisomerase II Mechanism.                                                         

 

6: Structure of Topoisomerase II.                                                         

 

7: Topoisomerase I Mechanism.                                                          

 

8: Structure of a Topoisomerase.                                                         

 

9: Topoisomers.                                                             

 

10: Linking Number.                                                             

 

11: DNA Polymerases Require a Template and a Primer                                

 

12: Conserved Residues among Helicases.                                                

 

13: Helicase Mechanism.                                                             

 

14: Helicase Structure.                                                             

 

15: Proofreading.                                                           

 

16: Shape Selectivity.                                                             

17: Minor-Groove Interactions.                                                         

 

18: DNA Polymerase Mechanism.                                                        

 

19: DNA Polymerase Structure.                                                         

 

20: DNA Can Assume a Variety of Structural Forms                                    

 

21: Comparison of A-, B-, and Z-DNA                                                   

 

22: Z-DNA.                                                          

 

23: Propeller Twist.                                                             

 

24: Major and Minor Grooves in B-Form DNA.                                          

 

25: Major- and Minor-Groove Sides.                                                     

 

26: Steric Clash.                                                          

 

27: Sugar Puckers.                                                            

 

28: B-Form and A-Form DNA.                                                         

 

29: Faithful copying is essential to the storage of genetic information.              

 

30: DNA Replication At Low Resolution.                                                

 

31: Consequences of Strand Separation.                                                  

 

32: DNA Replication.                                                            

 

33: DNA Replication, Recombination, and Repair                                       

 

34: Problems                                                         

35: Summary                                                        

 

36: Mutations Involve Changes in the Base Sequence of DNA                        

 

37: Ames Test.                                                         

 

38: Triplet Repeat Expansion.                                                           

 

39: Mismatch Repair.                                                             

 

40: Uracil Repair.                                                            

 

41: Excision Repair.                                                             

 

42: Structure of DNA-Repair Enzyme.                                                   

 

43: Repair Pathways.                                                             

 

44: Cross-Linked Dimer of Two Thymine Bases.                                         

 

45: Aflatoxin Reaction.                                                            

 

46: Acridines.                                                       

 

47: Chemical Mutagenesis.                                                            

 

48: Base Pair with 5-Bromouracil.                                                        

 

49: Base Pair with Mutagenic Tautomer.                                                  

 

50: Double-Stranded DNA Molecules with Similar Sequences Sometimes Recombine 
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