PCR Product Sequencing Protocols -- (Page 1, 2)
A reliable protocol for dsDNA and PCR product sequencing.
A reliable protocol
for dsDNA and PCR product sequencing. Cullmann G, Hubscher U, Berchtold MW.
www.ncbi.nlm.nih.gov/pubmed/8251152
Sequencing PCR Products Cloned into I\/I13 Vectors
method for sequencing PCR products cloned into M13 and plasmid vectors. ... sequencing
protocol. The first involves the annealing of the oligonucleotide ...
www.springerlink.com/index/g30w21t317757667.pdf
Production Protocol
for the EXO/SAP of Mutational Profiling PCR ...
PURPOSE: This protocol describes how to add EXO/SAP cocktail to PCR products on. the
Biomek FX to prepare them for sequencing. MATERIALS AND EQUIPMENT: ...
genome.wustl.edu/activity/med_seq/protocol/ProductionExoSapProtocol.pdf
Tech Tips: Direct Sequencing of PCR Products from an Agarose Gel ...
Modified protocol for direct sequencing of DNA from an agarose
gel using the ... Direct sequencing of a PCR product isolated using a plastic pipette tip (A) ...
www.epibio.com/f2_3/f2_3st.asp
A
genomic sequencing protocol that yields a positive display of 5 ...
A genomic sequencing protocol that yields a positive display of
... The PCR products can be sequenced directly to provide a strand-specific average sequence ...
www.pubmedcentral.nih.gov/articlerender.fcgi?artid=48546
Bisulfite-PCR For Restriction Analysis And/or Sequencing Protocol ...
Bisulfite-PCR For Restriction Analysis And or Sequencing Protocol.
... The PCR products are also suitable for either direct sequencing or cloning and ...
www.biocompare.com/protocols/protocol.asp?id=1891
Cycle Sequencing Protocol Cycle sequence is similar to PCR. It ...
Cycle Sequencing Protocol. Cycle sequence is similar to PCR. It
uses most of the same ingredients, ... PCR products into other parts of the lab. ...
www.wsu.edu/~drown/ProtocolSequencing.pdf
Improved
accuracy in direct automated DNA sequencing of small PCR ...
Using the Taq DNA polymerase dye terminator sequencing protocol and automated
sequencing apparatus from Applied BioSystems, 0.1 to 1.0 pmol of PCR product ...
www.ncbi.nlm.nih.gov/pubmed/7818900
Citation: A rapid
PCR based protocol to create GFP fusions
The protocol entails a simple fusion PCR, which fuses two primary PCR products ... markers
has any sort of sequence overlap to the co-injected PCR product, ...
www.wormbase.org/db/misc/paper?name=WBPaper00015760;class=Paper
- 13k -
Inverse PCR and Sequencing Protocol
Inverse PCR and Sequencing Protocol on 5 Fly Preps. For recovery of sequences flanking
XP elements. This protocol is an adaptation of ...
flystocks.bio.indiana.edu/pdfs/Exel_links/5__fly_iPCR_XP_pub.pdf
Optimized conditions
for cycle sequencing of PCR products.
stranded PCR products using the same. primers for PCR and sequencing was. needed. With such a
protocol, cycle se-. quencing can be directly applied to the ...
www.genome.org/cgi/reprint/3/6/359.pdf
GE Healthcare Life Sciences
- Sequenase PCR Product Sequencing Kit
Utilize enzymatic pretreatment to directly sequence PCR products without purification ... can
be sequenced directly using the standard Sequenase? protocol. ...
www5.gelifesciences.com/APTRIX/upp01077.nsf/Content/sequencing_site~sequencing_reaction~sequencing_reacti...
Quinteiro, J. and Rey-Mendez M. Dept. of Biochemistry and ...
PCR cycle sequencing protocol for population mitochondrial cytochrome
b analysis ... Sequencing PCR products with the Sequenasea PCR product sequencing kit. ...
www.usc.es/mtdna/doc/seqp.html
MultiPlex
PCR-based Sequencing
Sequencing:. Distribute 2 ul of the PCR products to each well of a Robbins 96 ... in 96 well microtiter plate
format (refer to the Roe Lab protocol book) ...
www.genome.ou.edu/MultiplexPCRbasedSeq.html
dilute the
PCR products with an equal volume of water (1:2 dilution); use 2-4 ul of the products for sequencing using the 1/8 BigDye dilution
protocol. ...
www.genome.ou.edu/uniplexPCRbasedseq.html
Sequencing Protocol for ABI 3730 Capillary Sequencer: 1ul Big ...
Sequencing
Protocol for ABI 3730 Capillary Sequencer:. 1ul Big Dyeterminator V3.1 ... for pcr products 20-50ng depending on size. ...
ramaciotti.science.unsw.edu.au/downloads/sequencing_reaction_protocol_AB3730_new.pdf
ECKER LAB PROTOCOL Nested PCR and Sequencing of T-DNA Junctions in ...
Load 6.5 ml of Ase pcr product on gel to visualize products.
... Precipitate the sequenced products using the EtOH/NaOAc protocol mentioned. previously. ...
signal.salk.edu/T-DNArecovery.pdf
Long-read
direct infrared sequencing of crude PCR products for ...
We present a complete protocol for direct sequencing of H 1 V-1 reverse transcriptase
... Direct use of the crude unpurified PCR product as the sequencing ...
cat.inist.fr/?aModele=afficheN&cpsidt=1637530
Direct DNA
sequencing of PCR products
kit protocol except that the times and temperatures were. changed. ... Shown on the left is a single-stranded
PCR product sequenced ...
doi.wiley.com/10.1002/em.2850180413
Springer Protocols: Full Text: Direct Sequencing of PCR Products ...
Springer
Protocols is the largest subscription-based electronic database of reproducible laboratory protocols in the Life and Biomedical Sciences.
www.springerprotocols.com/Full/doi/10.1385/0-89603-344-9:101?encCode=QkVDOjEwMTo5LTQ0My0zMDY5OC0w
Inverse PCR for PAC-end sequencing
Stages in protocol (1) Restriction digest of PAC DNA (2) ligation of restriction digests (3) PCR
amplification of ligation products (4) Sequencing of PCR ...
www.genetics.wustl.edu/fish_lab/frank/cgi-bin/fish/prot2.html
Preparing
Gel-Purified PCR Products for Sequencing ... Electrophorese these samples according to the instructions in Protocol 6. From the photograph
of the ...
www.mdibl.org/~dtowle/mbw/9.%20Sequencing.htm
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