Pat Brown's Laboratory
Stanford
National Human Genome Research Institute (NHGRI)
The Institute for Genomic Research (TIGR)
Microarray
Core Facility (Baylor College of Medicine)
Complete protocol for epoxy slides (QUANTIFOIL)
Complete protocol for aldehyde slides (QUANTIFOIL)
Complete
protocol for amino slides (QUANTIFOIL)
DNA Amplification and Purification
Preparation of DNA Samples (Brown Laboratory Stanford)
National
Human Genome Research Institute (NHGRI)
PCR Purification and Storage (TIGR)
Montage PCR Cleanup Filter Plates
(Millipore)
Slide Array Preparation & Printing
Poly-l-Lysine coating of slides (Brown Laboratory-Stanford)
Post-Processing Arrays
(Brown Laboratory-Stanford)
National Human Genome Research Institute (NHGRI)
Slide array preparation and printing
(QUANTIFOIL)
RNA Isolation
Fast Track mRNA Isolation (Brown Laboratory Stanford)
Preparation of Total RNA from Blood (Brown Laboratory-Stanford)
Poly A+ Isolation from Total RNA (TIGR)
FastTrack?2.0 mRNA Isolation Kit (Invitrogen)
Micro-FastTrack?2.0 mRNA Isolation
Kit (Invitrogen)
Technical Article (Ambion, Inc.)
Total RNA isolation kits and
reagents (Ambion, Inc.)
mRNA isolation kits and reagents (Ambion, Inc.)
ArrayIt Micro-Total RNA Extraction Kit (Telechem)
RNA Amplification
aRNA Amplification - Ambion Method (Brown Laboratory Stanford)
aRNA Amplification - Wang Method (Brown Laboratory Stanford)
aRNA
Amplification (Brown Laboratory Stanford)
Modified Eberwine RNA Amplification
Amino Allyl MessageAmp?aRNA Kit (Ambion, Inc.)
MessageAmp?II
aRNA Amplification Kit (Ambion, Inc.)
BDSmart mRNA Amplfication Kit (BD Biosciences Clontech)
ArrayIt MiniAmp mRNA Amplification Kit
(Telechem)
Probe Labeling
OU Microarray Core Facility (University of Oklahoma)
Labeling Yeast RNA (Brown Laboratory Stanford)
Labeling Human RNA (Brown Laboratory-Stanford)
LabelingE.Coli RNA (Brown Laboratory-Stanford)
Amino-Allyl Reverse Transcription (Brown Laboratory-Stanford)
Revised Amino-Allyl Labeling (Brown
Laboratory-Stanford)
Labeling by Reverse Transcription (NHGRI)
Amino-allyl Dye Coupling Protocol
Reverse Transcription/Labeling RNA
Modified
Eberwine RNA Amplification
Amino Allyl cDNA Labeling Kit (Ambion, Inc.)
FairPlay?II Microarray Labeling Kit
(Stratagene)
ArrayIt Indirect Amino Allyl Fluorescent Labeling Kit (Telechem)
ULS?labeling kits for DNA
microarray applications (Kreatech)
Microarray Target Purification Kit (Roche Biochemicals)
Label IT?µArray?Cy?/Cy? Labeling Kit (Mirus
Bio Corp.)
Hybridization & Washing
Array Hybridization-General/Yeast (Brown Laboratory-Stanford)
Array Hybridization-Human (Brown
Laboratory-Stanford)
Labeled Probe Hybridization (TIGR)
Hybridization/Washing Glass Slide Arrays (QUANTIFOIL)
Array
Washing Protocol (QUANTIFOIL)
Affymetrix GeneChips?Protocols
W.M. Keck Facility (Yale University)
Microarray Protocols
Basic knowledge of microarray.
Basic knowledge of microarray 2.
MicroArray Procedure
(Modified by Ryoji Kojima, Takumi Yoshida and Jeffrey Randall,
8.20.2000)
1. Total RNA Isolation from cultured cells.
2. DNase Treatment of Total RNA
3. Making the single strand
cDNA probe.
4. Automated Slide Processor (ASP) Version for hybridization.
5. Washing microarrays in ASP.
7. Pre-hybridization of the processed slides (NON-Automated version).
8. Hybridization of Cy3 + Cy5 probe
to glass array (NON-Automated version).
9. Preparation of Dendrimer Cy3 and Cy5.
10. Washing unbound probe from glass array
(NON-Automated version).
11. Hybridization of Dendrimers (Cy3 and Cy5) to Array (NON-Automated version).
12.Washing unbound
dendrimer from glass array(NON-Automated version).
Other Microarray Protocols (1)
Other Microarray Protocols (2)
Web Guider
Ch 8.Immunohistoch / immunology
Ch 10.GC/MS, NMR and Proteomics
