Other Online Microarray Protocols -- (1) (2) (3)
Fox Chase Cancer Center ADGE Microarray Protocol http://www.fccc.edu/docs/ADGE_Microarray/protocol.pdf. McArdle Laboratory
for Cancer Research cDNA EDGE ...
keck.med.yale.edu/dnaarrays/protocollinks.htm
research.nhgri.nih.gov/microarray/protocols.html
MICROARRAY PROTOCOL. Reagents & Equipments; Labelling; Purification; Hybridisation; Washing. News. Reagents
& Equipments. Oligo-dT (T15) - Roche ...
www.rbhrfcrc.qimr.edu.au/kidney/Pages/Microarray%20protocol.html
Microarray
Protocol. Reverse Transcription with fluorescent dye to make chip probes: Mix the following reactions for the IP and no IP amplification...
cmgm.stanford.edu/~kimlab/intestine/Microarray%20Protocol.htm
Invitrogen - Molecular Probes - An Optimized Protocol for Microarray QC with Alexa Fluor Dye-labeled Random Oligodeoxynucleotides.
probes.invitrogen.com/servlets/posters?id=488
HOME ?BACK ?PCR Amplification of cDNA inserts ?Microarray Hybridization Protocols ?Revisions and additional
needs.
microarray1k.aecom.yu.edu/protocol.html - 3k
Protein Lysate Microarray Protocol
Reverse Phase Protein Microarray Protocol ?Updated
2-26-06. PBST = 1x PBS pH 7.4 (no Ca or Mg) + 0.1% Tween-20 (make fresh) ...
utzlab.stanford.edu/publications/lysatemicroarray.doc
Ares Lab Microarray Protocol. Updated 7/2004. Page 2 of 5. RT Reaction. 1. Prepare reverse transcription master
mix:. 5x First
www.cbse.ucsc.edu/pdf_library/ares_lab_microarray_protocol072104.pdf
Microarray protocol
Microarray
protocol. SM Chambers, AA Merchant. RNAqueous. 1) Pellet cells and remove media. 2) Add 200 ul of Lysis/binding buffer from RNAqueous
kit. (STOP) ...
www.bcm.edu/labs/goodell/protocols/microarray.pdf
Microarray Protocols
Basic knowledge of microarray.
Basic knowledge of microarray 2.
MicroArray Procedure
(Modified by Ryoji Kojima, Takumi Yoshida and Jeffrey Randall,
8.20.2000)
1. Total RNA Isolation from cultured cells.
2. DNase Treatment of Total RNA
3. Making the single strand
cDNA probe.
4. Automated Slide Processor (ASP) Version for hybridization.
5. Washing microarrays in ASP.
7. Pre-hybridization of the processed slides (NON-Automated version).
8. Hybridization of Cy3 + Cy5 probe
to glass array (NON-Automated version).
9. Preparation of Dendrimer Cy3 and Cy5.
10. Washing unbound probe from glass array
(NON-Automated version).
11. Hybridization of Dendrimers (Cy3 and Cy5) to Array (NON-Automated version).
12.Washing unbound
dendrimer from glass array(NON-Automated version).
Other Microarray Protocols (1)
Other Microarray Protocols (2)
Web Guider
Ch 8.Immunohistoch / immunology
Ch 10.GC/MS, NMR and Proteomics
