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Ch 8.Immunohistoch / immunology
Ch 10.GC/MS, NMR and Proteomics
Stem Cell Research Protocols (1) (2)
Step by step protocol
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Human
Embryonic Stem Cell Culture
The following culture protocols provide detailed instructions for thawing and culturing both mEFs and hES
cells...
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Embryonic stem (ES) cells are totipotent cells derived from the inner cell
mass (ICM) of preimplantation mammalian...
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Rodent Neuron Differentiation Protocol
The protocol described in this
section utilizes the Rodent Neuron Differentiation Kit (Millipore Cat. No. SCR035). This system was designed...
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Isolation
of Primary Human Prostate Epithelium
A wide range of approaches exists for the isolation and growth of early passage prostate epithelium
cell cultures...
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Isolation of Human Corneal Epithelium
A wide range of approaches exists for the isolation and growth
of early passage corneal epithelium cell cultures...
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Differentiation of Cells in Progenitor Cell Targeted (PCT)
Media
As with all differentiation work, it is important that cells approach confluency before being induced to differentiate, for example
with calcium switch...
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Keratinocyte Differentiation Using PCT Media
Millipore's PCT Epidermal Keratinocyte Medium
is recommended for isolation and maintenance of keratinocyte...
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Establishment of 3-Dimensional Airway Epithelium
Cultures
Large airway epithelium cell cultures established and maintained in Millipore's PCT culture medium can be induced to...
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Mesenchymal Stem Cell Differentiation Protocols
Stem cell technology, particularly embryonic stem cells and/or mesenchymal
stem cells, offer attractive sources of adipocytes...
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Adipogenesis Differentiation Protocol
This protocol was developed
using Millipore's Mesenchymal Stem Cell Adipogenesis Kit, With this protocol, we typically obtain > 30%...
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WiCell
and The National Stem Cell Bank - FAQs for Introductory ...
The course focuses on precisely following our protocols. 7. What should
I bring with me to the course? ... Does this course teach feeder-free hES culture? ...
www.wicell.org/index.php?option=com_content&task=category§ionid=16&id=133&Itemid=176
Thomson
Lab Stem Cell Protocols
While the cells are spinning prepare the feeder layers by aspirating off the ... It is a good idea to learn
human ES cell culture on MEFs before moving to ...
ink.primate.wisc.edu/~thomson/protocol.html
Protocols