PCR Serials and Its Related Protocols
Regular PCR Procedure
Protocol for
PCR with Taq DNA Polymerase. Avoiding Contamination. PCR allows the production of more than 10 million copies of a target DNA sequence
from ...
www.fermentas.com/techinfo/pcr/dnaamplprotocol.htm -
Detailed PCR protocol from the web site of the Department
of Biology,
www.mcdb.lsa.umich.edu/labs/maddock/protocols/PCR/general_pcr_protocol.html
However,
efficient sequencing of dsDNA generated by normal PCR is possible using the modification to the SequenaseTM protocol published by
Bachmann et al. ...
www.mcb.uct.ac.za/pcrcond.htm
PCR PROTOCOL FOR cDNA ARRAYS ON MEMBRANES. Purpose: to amplify insert
DNA from purified plasmid DNA derived from bacterial. plasmid libraries. ...
www.daf.jhmi.edu/microarray/protocols/protocol6.pdf
Basic PCR Protocol. CGLab, 7/2002. 1). Wipe down the bench area with bleach and a new paper towel. 2). Take the PCR components
out of the freezer to thaw ...
www.sfsu.edu/~biology/cgl/media/PCR%20Protocol-Basic.pdf
Protocol and guidelines for
choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept.,
arep.med.harvard.edu/labgc/estep/longPCR_protocol.html
20-mer Polymerase Chain Reaction Procedure (for MJ Research ...
MJ Research thermal Cycler: 10-mer PCR for amplification of random
genomic DNA fragments ... Edit (or choose a program if it has been set up) PCR Program. ...
wheat.pw.usda.gov/~lazo/methods/lazo/pcrproto.html
Single tube confirmation PCR protocol
For characterization colonies of transformed clones of Saccharaomyces, from the web site of the
Stanford Genome Technology Center, Palo Alto, CA, USA.
www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html
Protocol
for PCR with Hot Start Taq DNA Polymerase
Protocol for PCR with Hot Start Taq DNA Polymerase. How to Avoid Contamination. During PCR,
usually more than 10 million copies of a template DNA can be ...
www.fermentas.com/profiles/modifyingenzymes/pdf/protocols/protocolhotstart.pdf
A
Basic Polymerase Chain Reaction Protocol
Here, a basic, straight-forward PCR protocol is. presented. Where appropriate, some of the
choices for modifying this standard reaction ...
www.idtdna.com/support/technical/TechnicalBulletinPDF/A_Basic_PCR_Protocol.pdf
PCR Reamplification for Inadequate or Failed Amplifications. Change your standard PCR protocol for the locus
as follows:. decrease the number of cycles by ...
genome-lab.ucdavis.edu/Protocols/pcr_tips/pcr_reamplification.htm
Inverse PCR and
Sequencing Protocol
Inverse PCR and Sequencing Protocol on 5 Fly Preps. For recovery of sequences flanking XP elements. This protocol
is an adaptation of ...
flystocks.bio.indiana.edu/pdfs/Exel_links/5__fly_iPCR_XP_pub.pdf
Web Guider
Ch 8.Immunohistoch / immunology
Ch 10.GC/MS, NMR and Proteomics