Commonly used stock solutions at Laboratory

 

0         30% Acrylamide (100 ml)

1        40% Acrylamide (for DNA sequencing)(1 liter)

2         M Adenosine triphosphate (ATP)(1 ml)

3        10 M Ammonium acetate (1 liter)

4         10% Ammonium persulfate (10 ml)

5        M CaCl2 (200 ml)

6         2.5 M CaCl2 (20 ml)

7        Deoxyribonucleoside triphosphates (dNTPs)

8         M Dithiothreitol (DTT)(20 ml)

9        0.5 M EDTA (pH 8.0)(1 liter)

10    Ethidium bromide (10 mg ml-1)(100 ml)

11    x HEPES-buffered saline (100 ml)

12    1 M Isopropylthio-?D-galactoside (IPTG)(10 ml)

13    1 M Magnesium acetate (MgOAc)(1 liter)

14    1 M MgCl2 (1 liter)

15    Phenol: chloroform

16    10 mM Phenylmethylsulfonyl fluoride (PMSF)(10 ml)

17    1 M Potassium acetate (pH 7.5)(100 ml)

18    M Sodium acetate (pH 7.0)(1 liter)

19    3 M Sodium acetate (pH 5.2)(1 liter)

20    M NaCl (1 liter)

21    10% Sodium dodecyl sulphate (SDS) (also called sodium lauryl sulphate)(1 liter)

22    20 x SSC (NaCl and sodium citrate)(1 liter)

23   20 x SSPE (Salt, sodium phosphate, EDTA)(1 liter)

24    Trichloroacetic acid (TCA) 100% solution (500 g TCA)

25   1 M Tris (1 liter)

26    Tris-buffered saline (TBS) (25mM Tris)(1 liter)

27    Tris-acetate for electrophoresis (TAE)(50X)(1 liter)

28    Tris-borate for electrophoresis (TBE)(5X)(1 liter)

29    Gel loading buffer Type I (with sucrose)(6X)(10 ml)

30    Gel loading buffer Type II (with Ficoll)(6X)(10 ml)

31    Gel loading buffer Type III (with glycerol)(6X)(10 ml)

32    Gel loading buffer Type IV (bromophenol blue and sucrose)(6X)(10 ml)

33    Gel loading buffer Type V (Alkaline loading buffer)(10 ml)

34    10X Phosphate buffered saline (10X PBS)(1 liter)

35    Microtubules stabilizing buffer (MTSB)(1 liter)

36    Ampicillin stock solution (25 mg ml-1)(40 ml)

37    Chloramphenicol stock solution (34 mg ml-1)(29.5 ml)

38    Kanamycin stock solution (25 mg ml-1)(40 ml)

39    Streptomycin stock solution (20 mg ml-1)(50 ml)

40    Tetracycline stock solution (12.5 mg ml-1)(80 ml)

41    Luria broth (1 liter)

42    M9 Salts (10X)(1 liter)

43   M9 Minimal medium (1 liter)

 

 

Commonly used solutions (1)---  1 ) 2 ) 3 ) 4 ) 5 ) 6 )

 

Commonly used solutions (2)--- 1 ) 2 ) 3 )

 

Solutions  for molecular cloning---1 ) 2 ) 3 ) 4 ) 5 ) 6 ) 7 ) 8 ) 9 )