commonly used solutions (2) ------ 1 ) 2 ) 3 )
10% or 20% (w/v) SDS. Also called sodium lauryl (or dodecyl) sulfate.
To prepare a 20% (w/v) solution, dissolve 200 g of electrophoresis-grade SDS in 900 ml of H2O. Heat to 68 ?C and stir with a magnetic
stirrer to assist dissolution. If necessary, adjust the pH to 7.2 by adding a few drops of concentrated HCl. Adjust the volume to
1 liter with H 2O. Store at room temperature. Sterilization is not necessary. Do not autoclave.
Use a mask when weighing this out.
20x
SSC
0.3M Na(3) citrate
3M NaCl
Bacto-tryptone 20 g
Yeast extract 5 g
NaCl 0.584 g
KCl 0.186 g Mix components
and adjust pH to 7.0 with NaOH and autoclave.
2 M Mg ++ stock:
MgCl 2-6H2O 20.33 g
MgSO 4 -7H2O 24.65 g
Distilled water to 100 ml.
Autoclave or filter sterilize.
2 M Glucose
Glucose 36.04 g
Distilled water to 100 ml. Filter sterilize.
For SOB Medium + magnesium:
Add 1 ml of 2 M Mg ++ stock to 99 ml SOB Medium.
For SOCMedium: Add 1 ml of 2 M Mg ++ stock and 1 ml of 2 M Glucose to 98 ml of SOB
Medium.
To prepare a 3 M solution: Dissolve 408.3 g of sodium acetate-3H2O in 800 ml of H 2O. Adjust the
pH to 5.2 with glacial acetic acid. Adjust the volume to 1 liter with H2O. Dispense into aliquots and sterilize by autoclaving.
Southern
Solutions:
Depurination solution (for Southern blotting)
250mM HCl
Denaturation solution (for Southern blotting)
1.5M NaCl
0.5M NaOH
Neutralization solution (for Southern blotting)
1.5M NaCl
0.5M Tris-HCl, pH adjusted to 7.5
Prepare a 50x stock solution in
1 liter of H2O:
242 g of Tris base
57.1 ml of glacial acetic acid
100 ml of 0.5 M EDTA (pH 8.0)
The 1x working solution is 40 mM Tris-acetate/1
mM EDTA.
Prepare a 5x stock solution in 1 liter of H2O:
54 g of Tris base
27.5 g of boric acid
20 ml of 0.5 M EDTA (pH
8.0)
The pH of the concentrated stock buffer should be approx. 8.3.. Some investigators prefer to use more concentrated stock solutions of TBE (10x as opposed to 5x). However, 5x stock solution is more stable because the solutes do not precipitate during storage. Passing the 5x or 10x buffer stocks through a 0.22 um filter can prevent or delay formation of precipitates.
Commonly used solutions (1)--- 1 ) 2 ) 3 ) 4 ) 5 ) 6 )
Commonly used solutions (2)--- 1 ) 2 ) 3 )
Solutions for molecular
cloning---1 ) 2 ) 3 ) 4 ) 5 ) 6 ) 7 ) 8 ) 9 )
Web Guider
Ch 8.Immunohistoch / immunology
Ch 10.GC/MS, NMR and Proteomics